Features of pBR322
Asked by Mishka Mistri · 2 years ago
What are the key features of pBR322 that make it useful for genetic engineering?
1 Answer
pBR322 has several key features that make it useful for genetic engineering:
- Antibiotic Resistance Genes: It contains genes for resistance to ampicillin (ampR) and tetracycline (tetR), which allow for the selection of transformed cells.
- Multiple Cloning Sites: pBR322 has multiple unique restriction sites that enable the insertion of foreign DNA fragments.
- Origin of Replication: It includes an origin of replication (ori) that allows it to replicate independently within a bacterial cell.
- Small Size: The small size of pBR322 (around 4,361 base pairs) makes it easy to manipulate and transfer into bacterial cells.
NEET Faculty · 2 years ago
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