In C4 plants, carboxylation is twice, it can be represented as
In C4 plants, carboxylation occurs in two steps. First, PEP (phosphoenolpyruvate) combines with CO2 to form oxaloacetic acid, which is then converted to malate. The malate is transported to bundle-sheath cells, where it releases CO2 to be fixed again by RuBP (ribulose bisphosphate) in the Calvin cycle.