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NEET Practice Questions (MCQs) with Answers & Solutions

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All Physics Chemistry Botany Zoology

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E.coli is a commonly used host for gene cloning because

It is free from elements that interfere with replication and recombination of DNA

It is easy to transform

It supports replication of inserted DNA.

All of these

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Explanation

NCERT pg 199

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An ideal plasmid for recombinant technology DNA technology

A. Has maximum amount of DNA B. Has relaxed replication control C. Many recognition sites for one RE D. All of these

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Explanation

NCERT pg199

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Electroporation facilitates insertion of foreign DNA into the cell by

Changing the porocity of the membrane

Changing the electrical potential of the membrane

Lysing the cell wall

Active transport

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Explanation

The membranes are not able to pass electric current and hence act as capacitors. A high voltage current results in their temporary breakdown creating pores big enough through which macromoleclues can pass. Electroporation is 10 times more effective than chemicalmethods.

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The restriction enzymes most commonly used in rDNA technology are

Type 1 Restriction enzymes

Type II Restriction enzymes

Type III Restriction enzymes

Type IV Restriction enzymes

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Explanation

Type I RE-Cleave randomly far from their recognition sites.

Type II RE- Cleave within or close to their recognition sites

Type III RE – Require two recognition sites in opposite orientation and cleave outsid e the recognition site

Type IV RE- Cleave Methylated/Modified DNA sites.

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A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?

Southern blotting and South Western blotting

Northern blotting and western blotting

Western blotting and Eastern blotting

Southern and Northern blotting

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Explanation

Southern blot- detecting DNA

Northern blot- for detecting RNA

Western blot- for detecting Proteins

Eastern Blot- for detecting Protein post translational Modifications

Southwestern Blot-for detecting DNA binding proteins.

Northwestern Blot –for detecting RNA Protein interactions

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What is true about the DNA polymerase used in PCR?

A. Isolated from a virus B. Stable at high temperatures C. Used as selectable marker D. Has methylated bases

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Explanation

NCERT pg 203

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A reporter enzyme used in biotechnology is-

A. Green Fluorescent Protein B. Luciferase C. b Galactosidase D. All of the above

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Explanation

A reporter protein helps to detect recombinants through visual markers.

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Genetic engineering is possible because-

A. DNA can be cut at specific sites by using enzymes like DNAase II B. Restriction enzymes can be used in vitro C. DNA ligase can join two pieces of DNA D. E.coli acts as an ideal host for cloning.

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Explanation

NCERT pg194-195

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Which among the following is a cloning vector?

A. EcoRI B. BamHI C. pUC18 D. Sal I

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Explanation

The rest are cloning sites

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Two bacteria found to be very useful in genetic engineering are

A. Diplococcus and Streptomyces B. Rhizobium and Xanthomonas C. Vibrio and Escherischia D. Escherischia and Agrobacterium

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Explanation

NCERT pg 200

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