Which of the following events correctly describes the chromosome number and DNA content during Anaphase in a mitotic cell division from a diploid organism (2n chromosomes, 2C DNA content in G1)?
At the start of mitosis (after S phase), a diploid cell has 2n chromosomes, each with two chromatids, and 4C DNA content. During anaphase, the centromeres split, and sister chromatids separate, moving to opposite poles. Each separated chromatid is now considered an individual chromosome. Therefore, temporarily, the chromosome number doubles to 4n (each pole receives 2n chromosomes), while the total DNA content within the dividing cell is still 4C (before cytokinesis is complete).