NEET Practice Questions (MCQs) with Answers & Solutions

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Consider the following statements regarding protein denaturation:
I. The overall folding of the polypeptide chain is affected.
II. The sequence of amino acids changes.
III. Intermolecular hydrogen bonds are strengthened.
Which of the statements is/are correct?

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Explanation

I is correct because 'globules unfold and helix get uncoiled' affecting overall folding (tertiary and secondary structures). II is incorrect because the primary structure (amino acid sequence) remains intact. III is incorrect because hydrogen bonds are 'disturbed', not strengthened.

Which of the following conditions is LEAST likely to cause denaturation of a protein?

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Explanation

Denaturation is caused by changes in temperature or pH that disturb hydrogen bonds. Maintaining optimum conditions (temperature and pH) is where a protein exhibits its highest activity and is least likely to denature. Extremes of temperature and pH (like strong acids/bases leading to extreme pH) cause denaturation.

Which of the following organisms exhibit RNA interference (RNAi) as a method of cellular defense?

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Explanation

The NCERT text states, 'RNAi takes place in all eukaryotic organisms as a method of cellular defense.'

The nematode Meloidegyne incognitia infects which part of the tobacco plant, leading to a reduction in yield?

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Explanation

The NCERT book mentions, 'A nematode Meloidegyne incognitia infects the roots of tobacco plants and causes a great reduction in yield.' This highlights the specific target of the nematode.

In RNA interference (RNAi), what is the key molecule directly responsible for silencing a specific mRNA?

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Explanation

The NCERT text states, 'This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).'

What is the primary role of the complementary dsRNA molecule in RNA interference (RNAi)?

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Explanation

According to the NCERT text, the dsRNA 'binds to and prevents translation of the mRNA (silencing).'

Which agricultural biotechnology technique is employed to introduce nematode-specific genes into host plants to achieve pest resistance via RNAi?

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Explanation

The context clearly states, 'Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant...'

In the RNAi-based pest resistance strategy for tobacco plants, the introduced DNA was designed to produce which types of RNA in the host cells?

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Explanation

The NCERT text explains, 'The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells.' These then form dsRNA.

What is the consequence for the nematode parasite when a transgenic host plant expresses specific interfering RNA through RNAi?

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Explanation

The NCERT states, 'The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA.'

The source of complementary RNA that can initiate RNAi can be from:

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Explanation

The NCERT text provides, 'The source of this complementary RNA could be from an infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate.'

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