RNA interference (RNAi) is described as a method of cellular defense common to:
The context mentions: 'RNAi takes place in all eukaryotic organisms as a method of cellular defense.'
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RNA interference (RNAi) is described as a method of cellular defense common to:
The context mentions: 'RNAi takes place in all eukaryotic organisms as a method of cellular defense.'
What is the primary consequence of specific mRNA silencing in the nematode Meloidogyne incognitia when it attempts to infect a transgenic host plant producing interfering RNA?
The text states: 'The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant therefore got itself protected from the parasite.'
In the RNAi strategy for pest resistance in tobacco plants, which vector was used to introduce nematode-specific genes into the host plant?
The context mentions: 'Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant.'
The double-stranded RNA (dsRNA) molecule, essential for initiating RNAi, is formed in the host plant cells by the combination of:
The text explains: 'The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi...'
Beyond viral infections, what other potential source of complementary RNA is mentioned that could initiate RNAi in eukaryotic organisms?
The context states: 'The source of this complementary RNA could be from an infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate.'
What is the main advantage of using genetically modified (GM) crops, specifically pest-resistant plants, as mentioned in the context?
The text highlights that GM plants have 'reduced reliance on chemical pesticides (pest-resistant crops)' and 'could decrease the amount of pesticide used'.
The phenomenon of RNA interference (RNAi) in pest resistance is based on what fundamental molecular process?
The context defines RNAi as a method that 'involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).'
Which of the following is NOT a direct application of genetic modification mentioned in the context for improving crop characteristics?
The context mentions tolerance to 'abiotic stresses (cold, drought, salt, heat)' and not just cold temperatures. All other options are direct applications mentioned.
The 'T-DNA' transferred by Agrobacterium tumifaciens to plant cells typically results in:
Agrobacterium tumifaciens 'is able to deliver a piece of DNA known as ‘T-DNA’ to transform normal plant cells into a tumor and direct these tumor cells to produce the chemicals required by the pathogen.'
Why did the RNAi-based strategy for Meloidogyne incognitia infeststion in tobacco involve the production of both sense and anti-sense RNA?
The context states: 'The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi...'
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