The primary cause for the dilution of filtrate as it moves up the ascending limb of Henle's loop is:
The text notes: 'The filtrate gets concentrated as it moves down the descending limb but is diluted by the ascending limb.' The dilution occurs because the ascending limb is impermeable to water, but allows electrolytes to be transported out (passively in the thin part, actively in the thick part), thus reducing the solute concentration of the filtrate without water following it.