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Electroporation facilitates insertion of foreign DNA into the cell by
The membranes are not able to pass electric current and hence act as capacitors. A high voltage current results in their temporary breakdown creating pores big enough through which macromoleclues can pass. Electroporation is 10 times more effective than chemicalmethods.
The restriction enzymes most commonly used in rDNA technology are
Type I RE-Cleave randomly far from their recognition sites.
Type II RE- Cleave within or close to their recognition sites
Type III RE – Require two recognition sites in opposite orientation and cleave outsid e the recognition site
Type IV RE- Cleave Methylated/Modified DNA sites.
A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?
Southern blot- detecting DNA
Northern blot- for detecting RNA
Western blot- for detecting Proteins
Eastern Blot- for detecting Protein post translational Modifications
Southwestern Blot-for detecting DNA binding proteins.
Northwestern Blot –for detecting RNA Protein interactions
What is true about the DNA polymerase used in PCR?
NCERT pg 203
A reporter enzyme used in biotechnology is-
A reporter protein helps to detect recombinants through visual markers.
Genetic engineering is possible because-
NCERT pg194-195
Which among the following is a cloning vector?
The rest are cloning sites
Two bacteria found to be very useful in genetic engineering are
NCERT pg 200
Which of the following is a recombinant protein-
NCERT pg 203
An example of a transgenic is
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