Which of the following is used to make vaccines?
(c) : Attenuation is the lessining or reduction of virulence of a pathogenic bacterium or virus. The phenomenon was first noticed by Louis Pasteur (1822-95), who developed it as a means of producing strains of bacteria suitable for vaccination. Attenuated strains of bacteria are obtained by successive subculturing in controlled conditions, whereas attenuated virus may be obtained by ultraviolet irradiation. Such strains of bacteria or viruses are used to induce active immunity by inoculation.