Practice free Zoology NEET multiple-choice questions online with instant answers and detailed explanations. No login required.
What is considered one of the best methods for couples looking for parenthood, especially given the large number of orphaned and destitute children in India?
The NCERT highlights, 'Our laws permit legal adoption and it is as yet, one of the best methods for couples looking for parenthood.'
Embryos formed by in-vivo fertilisation can also be used for transfer. Which type of transfer would be appropriate for an embryo with more than 8 blastomeres formed this way into a female who cannot conceive?
The text states, 'Embryos formed by in-vivo fertilisation (fusion of gametes within the female) also could be used for such transfer to assist those females who cannot conceive.' And for embryos with more than 8 blastomeres, IUT (intra uterine transfer) is used.
Which of the following describes the function of an 'origin of replication' (ori) in a cloning vector?
The NCERT text states: 'Origin of replication (ori) : This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA.'
A recombinant plasmid containing a foreign DNA fragment inserted within the tetracycline resistance gene of pBR322 is transformed into E. coli. Which of the following statements correctly describes the selection of recombinants?
The NCERT text mentions: 'The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes. For example, you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector pBR322. The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from non-recombinant ones by plating the transformants on ampicillin containing medium.'
Which of the following is NOT a characteristic feature required in a cloning vector?
The NCERT text states: 'In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.'
In the process of insertional inactivation using $\beta$-galactosidase, how are recombinant colonies identified?
The NCERT text explains: 'The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the $\beta$-galactosidase gene and the colonies do not produce any colour, these are identified as recombinant colonies.'
Which of the following pathogens is used to deliver genes into dicot plants because of its natural gene transfer ability?
The NCERT text mentions: 'For example, Agrobacterium tumifaciens, a pathogen of several dicot plants is able to deliver a piece of DNA known as ‘T-DNA’ to transform normal plant cells into a tumor...'
A selectable marker in a cloning vector serves which of the following purposes?
The NCERT text states: 'Selectable marker : In addition to ‘ori’, the vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.'
Which of the following antibiotic resistance genes are commonly used as selectable markers for E. coli?
According to the NCERT text: 'Normally, the genes encoding resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli.'
What is the primary reason that bacteriophages are considered useful as cloning vectors?
The NCERT text states: 'Bacteriophages because of their high number per cell, have very high copy numbers of their genome within the bacterial cells.'
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