Zoology MCQs for NEET — Practice Questions with Answers

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In the RNAi strategy for pest resistance in tobacco plants, which vector was used to introduce nematode-specific genes into the host plant?

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Explanation

The context mentions: 'Using Agrobacterium vectors, nematode-specific genes were introduced into the host plant.'

The double-stranded RNA (dsRNA) molecule, essential for initiating RNAi, is formed in the host plant cells by the combination of:

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Explanation

The text explains: 'The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi...'

Beyond viral infections, what other potential source of complementary RNA is mentioned that could initiate RNAi in eukaryotic organisms?

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Explanation

The context states: 'The source of this complementary RNA could be from an infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate.'

What is the main advantage of using genetically modified (GM) crops, specifically pest-resistant plants, as mentioned in the context?

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Explanation

The text highlights that GM plants have 'reduced reliance on chemical pesticides (pest-resistant crops)' and 'could decrease the amount of pesticide used'.

The phenomenon of RNA interference (RNAi) in pest resistance is based on what fundamental molecular process?

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Explanation

The context defines RNAi as a method that 'involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing).'

Which of the following is NOT a direct application of genetic modification mentioned in the context for improving crop characteristics?

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Explanation

The context mentions tolerance to 'abiotic stresses (cold, drought, salt, heat)' and not just cold temperatures. All other options are direct applications mentioned.

The 'T-DNA' transferred by Agrobacterium tumifaciens to plant cells typically results in:

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Explanation

Agrobacterium tumifaciens 'is able to deliver a piece of DNA known as ‘T-DNA’ to transform normal plant cells into a tumor and direct these tumor cells to produce the chemicals required by the pathogen.'

Why did the RNAi-based strategy for Meloidogyne incognitia infeststion in tobacco involve the production of both sense and anti-sense RNA?

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Explanation

The context states: 'The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA’s being complementary to each other formed a double stranded (dsRNA) that initiated RNAi...'

What specific type of genetic engineering tool from bacteria and viruses did scientists learn from to transfer genes into plants and animals?

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Explanation

The text says: '...we have learnt the lesson of transferring genes into plants and animals from bacteria and viruses which have known this for ages – how to deliver genes to transform eukaryotic cells and force them to do what the bacteria or viruses want.'

The use of baculoviruses as biological control agents for insect pests is considered desirable for integrated pest management (IPM) programs primarily because:

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Explanation

The context states, 'The majority of baculoviruses used as biological control agents are in the genus Nucleopolyhedrovirus. These viruses are excellent candidates for species-specific, narrow spectrum insecticidal applications. They have been shown to have no negative impacts on plants, mammals, birds, fish or even on non-target insects.'

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