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Biotechnology and its Applications MCQs for NEET — Zoology Questions with Answers

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A protein is not expressed properly in a diseased tissue. To find out whether the defect is at the level of translation or post translational modifications which techniques would you use ?

Southern blotting and South Western blotting

Northern blotting and western blotting

Western blotting and Eastern blotting

Southern and Northern blotting

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Explanation

Southern blot- detecting DNA

Northern blot- for detecting RNA

Western blot- for detecting Proteins

Eastern Blot- for detecting Protein post translational Modifications

Southwestern Blot-for detecting DNA binding proteins.

Northwestern Blot –for detecting RNA Protein interactions

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What is true about the DNA polymerase used in PCR?

A. Isolated from a virus B. Stable at high temperatures C. Used as selectable marker D. Has methylated bases

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Explanation

NCERT pg 203

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A reporter enzyme used in biotechnology is-

A. Green Fluorescent Protein B. Luciferase C. b Galactosidase D. All of the above

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Explanation

A reporter protein helps to detect recombinants through visual markers.

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Genetic engineering is possible because-

A. DNA can be cut at specific sites by using enzymes like DNAase II B. Restriction enzymes can be used in vitro C. DNA ligase can join two pieces of DNA D. E.coli acts as an ideal host for cloning.

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Explanation

NCERT pg194-195

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Which among the following is a cloning vector?

A. EcoRI B. BamHI C. pUC18 D. Sal I

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Explanation

The rest are cloning sites

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Two bacteria found to be very useful in genetic engineering are

A. Diplococcus and Streptomyces B. Rhizobium and Xanthomonas C. Vibrio and Escherischia D. Escherischia and Agrobacterium

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Explanation

NCERT pg 200

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Which of the following is a recombinant protein-

A. b Galactosidase in E.coli B. Insulin in humans C. Somatotropin in humans D. Interferon in E. coli

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Explanation

NCERT pg 203

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An example of a transgenic is

A. E.coli expressing b Galactosidase gene B. Carrot expressing Nif genes C. HIV expressing Reverse transcriptase gene D. Brassica expressing erusic acid gene

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Which of the following would you choose as the safest and least cumbersome selectable marker-

A. Ampicillin resistance gene B. Tetracyclin resistance gene C. Kanamycin resistance gene D. b Galactosidase gene

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Explanation

NCERT pg. 200

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Recombinant DNA is formed in nature during-

A. Crossing over at pachytene B. Integration of plasmid to form episome C. Integration of phage DNA to form prophage D. More than one is correct.

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Explanation

In all these processes DNA from two different sources combine to form Recombinant DNA

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