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Commonly consumed rice varieties has ……. Iron content of Iron fortified rice variety.
NCERT page number -176
9.2.4 Plant Breeding for Improved Food Quality
Strategies For Enhancement In Food Production
A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA
was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :
Both genes are functional in non-recombinants. In recombinants the gene for tetracycline resistance would be insertionally inactivated.
Elution is:
Answer 3. Read page 198, XII NCERT
A host cell normally does not take up a foreign DNA until it has been made competent to do so. This is because:
Cell membrane is made of a phospholipid bilayer and allows only lipid soluble substances to diffuse across it.
The first type II restriction endonuclease discovered that could cut dsDNA specific site was:
Answer 3. read page 195, XII NCERT
A gene, whose expression helps to identify transformed cells is known as
(a) A gene whose expression helps to identify transformed cell is known as selectable marker. Usually, the genes encoding resistance to antibiotics, such as tetracycline, ampicillin, etc. are useful selectable markers for e.g E.Coli.
Concept Enhancer Plasmid pBR322 has two resistance genes; amphicillin resistance (ampR) and tetracyclin resistance (tetR). These are considered as useful selectable markers.
The DNA fragments separated on an agarose gel can be visualised after staining with
(d) The DNA fragments separated on an agarose gel can be visualised after staining with ethidium
bromide. It is intercalating agent and a fluorescent agent. The stained DNA fragments are seen
bright orange coloured band under UV-light.
Thinking process Intercalation is the insertion of molecules between the planar bases of DNA. This
process is used as a method for analysing DNA. Intercalation occurs, when ligands of an appropriate
size and chemical nature fit themselves in between base pairs of DNA. These ligands are mostly
polycyclic, aromatic and planar and therefore often make good nucleic acid stains. Intensively
studied DNA intercalator include ethidium bromide, proflavine, etc.
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
(d) DNA ligases (genetic gum) are used in recombinant DNA technology to join two individual fragments of double-stranded DNA by forming phosphodiester bonds between them to produce a recombinant DNA (plasmid).
Which of the following restriction enzymes produces blunt ends?
The restriction enzyme EcoRV recognizes the palindromic DNA sequence 5'-GATATC-3' and cleaves it to produce blunt ends (no overhanging bases). Other enzymes like SalI, XhoI, and HindIII produce sticky ends (overhanging bases) after cleaving DNA.
Which of the following is a endonuclease?
(d) I-Iind II is a restriction endonuclease. Restriction endonucleases are enzymes used for cutting of
DNA at specific locations.
Hind II was the first restriction endonuclease isolated by Smith Wilcox and Kelley in 1968. It was
found that it always cut DNA molecules at a particular point by recognising a specific sequence of six
base pairs.
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