Practice free Molecular Basis of Inheritance (Botany) NEET multiple-choice questions online with instant answers and detailed explanations. No login required.
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:
Chilled ethanol selectively precipitates DNA as fine threads.
In gene gun method used to introduce alien DNA into host cells, microparticles of _____ metal are used.
Biolistic gun uses tungsten or gold microparticles coated with DNA.
Upon exposure to UV radiation, DNA stained with ethidium bromide will show:
EtBr-stained DNA fluoresces bright orange under UV.
What is the role of RNA polymerase III in the process of transcription in Eukaryotes?
RNA Pol I → large rRNAs, Pol II → hnRNA/mRNA, Pol III → tRNA + 5S rRNA + snRNAs.
Expressed Sequence Tags (ESTs) refers to:
ESTs are short sequences from mRNA → represent expressed (protein-coding) genes.
Unequivocal proof that DNA is the genetic material was first proposed by:
Hershey-Chase blender experiment (1952) — unequivocal proof.
How many different proteins does the ribosome consist of?
Eukaryotic 80S ribosome contains approximately 80 distinct proteins (NCERT figure).
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence:
A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR.
Isolate → Cut → Amplify → Insert: C, B, D, A.
The lactose present in the growth medium of bacteria is transported to the cell by the action of:
lac operon's $\beta$-galactoside permease (lacY) transports lactose into the cell.
What is the fate of a piece of DNA carrying only gene of interest which is transferred into an alien organism?
A. The piece of DNA would be able to multiply itself independently in the progeny cells of the organism.
B. It may get integrated into the genome of the recipient.
C. It may multiply and be inherited along with the host DNA.
D. The alien piece of DNA is not an integral part of chromosome.
E. It shows ability to replicate.
Choose the correct answer from the options given below:
A naked piece of DNA without ori cannot replicate independently — it must integrate into the host genome (B) and be inherited with it (C).
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