Biotechnology Principles and Processes MCQs for NEET — Zoology Questions with Answers

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Which of the following is not concerned with biotechnology?

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Explanation

Wood seasoning is not concerned with biotechnology. Biotechnology involves the use of living organisms or systems to develop or make products, whereas wood seasoning is a process of drying wood to remove moisture content.

The enzymes that cuts specifically recognition sites in the DNA is known as

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Explanation

Restriction endonucleases are enzymes that cut DNA at specific recognition sites. These enzymes are essential tools in molecular biology for gene cloning and DNA analysis.

Restriction endonuclease is also known as -

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Explanation

Restriction endonucleases are enzymes that cut DNA at specific recognition nucleotide sequences known as restriction sites. They are often referred to as molecular scissors because they cleave the DNA at these specific sites, creating fragments that can be manipulated for various genetic experiments.

Ideal host for the amplification of DNA molecules is

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Explanation

Bacteria are ideal for the amplification of DNA because of 2 reasons; their short doubling time; and availability extensive knowledge and tools for manipulating the bacteria. The short doubling time is advantageous because it will provide enough amounts of amplified DNA (of our interest) in a short span of time.

Ti Plasmid naturally occurs in

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Explanation

The Ti plasmid (Tumor-inducing plasmid) is a type of plasmid that naturally occurs in the bacterium Agrobacterium tumefaciens. This plasmid is responsible for transferring genetic material from the bacterium to the plant, causing crown gall disease. Scientists use this ability to introduce new genes into plants in genetic engineering.

Which of the following are the essential requirements for recombination ?

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Explanation

For recombination to occur, DNA ligase is essential. DNA ligase is an enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. This is crucial for the ligation of DNA fragments during the recombination process.

Ti Plasmid is useful in

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Explanation

Ti Plasmid (Tumor-inducing Plasmid) is derived from the bacterium Agrobacterium tumefaciens and is commonly used in genetic engineering to transfer new genes into plant cells. It has the ability to integrate a segment of its DNA (T-DNA) into the plant genome, making it a powerful tool for plant genetic modification.

Many copies of a DNA molecule in a test tube are procurred by

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Explanation

Polymerase Chain Reaction (PCR) is a widely used technique in molecular biology to amplify a single or a few copies of a DNA segment into millions of copies. This is achieved by using a DNA polymerase enzyme to replicate the DNA in vitro, making it a crucial method for DNA analysis and research.

Which of the following is used in genetic engineering ?

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Explanation

Restriction endonucleases are enzymes that cut DNA at specific sequences, which is a crucial step in genetic engineering. These enzymes create recombinant DNA molecules by cutting and rejoining DNA fragments from different sources.

Which one of the following pairs is correctly matched ?

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Explanation

Restriction enzymes are crucial tools in genetic engineering as they cut DNA at specific sequences, allowing scientists to manipulate and study genes. This makes the pair correctly matched.

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