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Biotechnology Principles and Processes NEET Questions — Free MCQ Practice

Practice free Biotechnology Principles and Processes (Zoology) NEET multiple-choice questions online with instant answers and detailed explanations. No login required.

NEET 2025

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Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer:

A. Both statement I and statement II are correct B. Both statement I and statement II are incorrect C. Statement I is correct but statement II is incorrect D. Statement I is incorrect but statement II is correct

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Explanation

Eluted DNA fragments can be used in rDNA construction (I correct). In agarose gel, smaller fragments travel farther toward the anode while larger ones stay near the wells (II correct).

NEET 2025

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Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer:

A. C and D only B. A and B only C. D and E only D. B and C only

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Explanation

Restriction enzymes, DNA ligase and DNA polymerase are essential for gene cloning. DNA mutase (C) and DNA recombinase (D) are not required.

NEET 2025

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The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method:

Statement I: The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies.

Statement II: The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies.

In the light of the above statements, choose the most appropriate answer:

A. Both Statement I and Statement II are correct B. Both Statement I and Statement II are incorrect C. Statement I is correct but Statement II is incorrect D. Statement I is incorrect but Statement II is correct

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Explanation

Insertion of DNA inactivates the lacZ gene, so recombinants cannot make the blue colour and form white colonies. Hence Statement II is correct and Statement I is incorrect.

Biotechnology Principles and Processes MCQs for NEET — Zoology Questions with Answers

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